白玉兰落叶水浸出液抑制蓝藻生长和叶绿素荧光特性分析
Effects of aqueous extract of Magnolia denudata defoliation on the growth and chlorophyll fluorescence characteristics of cyanobacterium
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摘要: 利用浮游植物荧光仪对暴露于不同浓度白玉兰落叶水浸出液下微囊藻生长、最大光合作用效率(Fv/Fm)、实际光合作用效率、光能利用效率(alpha)和最大相对电子传递速率(rETRmax)进行为期15 d的检测, 分析白玉兰落叶浸出液对微囊藻的抑制效应和叶绿素荧光特性影响.结果发现, 白玉兰落叶浸出液能有效抑制微囊藻的生长, 呈明显浓度抑制型变化, 抑藻能力随时间的延长而下降.低浓度(0.4、0.8、1.2、1.6 g·L-1)浸出液胁迫下, 对微囊藻叶绿素荧光参数无显著影响;高浓度(2.0 g·L-1) 浸出液胁迫下, 在早期(4 d内) 对荧光参数有极显著抑制作用.三维荧光图谱表明, 在投量为2.0 g·L-1时, 第15天色氨酸及酪氨酸荧光峰强度约为1.2 g·L-1投量情况下的1/3, 同时腐殖酸的荧光峰强度减弱.第7—15天, 藻细胞生长的半抑制浓度EC50值最小约为0.5—0.7 g·L-1.Abstract: The growth and physiology of bloom-forming M. aeruginosa were determined by the pulse amplitude modulated fluorimetry when exposed to aqueous extract of M. denudata defoliation for 15 d. The cell growth, maximal efficiency (Fv/Fm), effective quantum yield (Y(Ⅱ)) of PSⅡ photochemistry, photosynthesis efficiency (alpha) and maximum relative electron transport rate (rETRmax) were used to evaluate the growth and photosynthesis in M. aeruginosa. The extract stored for 5 days significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. It showed that low concentrations (0.4, 0.8, 1.2 and 1.6 g·L-1) of the extract had little effect on the four chlorophyll fluorescence parameters. However, high concentration (2.0 g·L-1) of the extract significantly affected the fluorescence parameters at early stage (4 d). Three-dimensional fluorescence spectroscopy showed that on day 15 of the treatment the peak intensity of tryptophan and tyrosine fluorescence with 2.0 g·L-1 extract is about a third of than 1.2 g·L-1 extract. At the same time, the peak intensity of humic acid fluorescence was much weaker. On 7—15 d, the EC50 of algal growth was the smallest which was about 0.5—0.7 g·L-1.
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