Pb、Zn及其交互作用对蜈蚣草抗氧化酶及叶绿素含量的影响
Effect of Pb and Zn interaction on antioxidant enzymes and chlorophyll content of Pteris vittata
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摘要: 采用水培实验的方法,研究了Pb、Zn及其交互作用对蜈蚣草抗氧化酶及叶绿素含量的影响.实验结果显示,Pb浓度在0-50 mg·L-1时,蜈蚣草叶绿素含量随Zn浓度增加逐渐升高、MDA含量逐渐降低,Pb、Zn交互作用可促进植株叶绿素合成.Pb浓度在100-400 mg·L-1时,Pb、Zn交互作用则降低叶绿素含量;在Pb浓度400 mg·L-1时,Pb、Zn交互作用显著增加MDA含量.实验结果还显示,在Pb浓度固定时,蜈蚣草叶片SOD、CAT的活性随Zn浓度增加呈现先上升后下降的趋势;POD随着Zn处理浓度的增加持续增加.在Zn浓度一定时,蜈蚣草叶片SOD、CAT、POD的活性随Pb浓度增加先上升后下降.由此可见,在低Pb、Zn浓度下,Pb、Zn交互作用表现为协同作用;在高Pb、Zn浓度下,Pb、Zn交互作用表现为拮抗作用.Abstract: The effect of Pb and Zn interaction on antioxidant enzymes and chlorophyll content in Pteris vittata were evaluated by the nutrient solution culture experiments. The results showed that when Pb concentration was between 0-50 mg·L-1, chlorophyll content of Pteris vittata gradually increased and MDA content decreased with increasing Zn concentrations. Pb and Zn interaction promoted the synthesis of chlorophyll. When the Pb concentration was between 100-400 mg·L-1, Pb and Zn interaction decreased chlorophyll content. When the Pb concentration was 400 mg·L-1,the interaction increased MDA content significantly.The experimental results also showed that when Pb concentration was fixed, SOD and CAT activities in Pteris vittata increased first and then decreased with increasing Zn concentrations, and POD activity increased continuously. When Zn concentration was fixed, SOD,CAT and POD activities increased first and then decreased with increasing Pb concentration. It can be seen that at low Pb and Zn concentrations, their interaction presented synergistic,while at high concentrations,they showed antagonistic effect.
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Key words:
- Pb /
- Zn /
- interaction /
- Pteris vittata /
- antioxidant enzymes /
- chlorophyll
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