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巩宁, 孟紫强, 邵魁双, 孙野青. 模式生物大型溞在水生生态毒理实验教学中的应用[J]. 生态毒理学报, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
引用本文: 巩宁, 孟紫强, 邵魁双, 孙野青. 模式生物大型溞在水生生态毒理实验教学中的应用[J]. 生态毒理学报, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
shu
Gong Ning, Meng Ziqiang, Shao Kuishuang, Sun Yeqing. Application of Water Flea in Experimental Teaching of Aquatic Ecotoxicology Based on Daphnia magna[J]. Asian journal of ecotoxicology, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
Citation: Gong Ning, Meng Ziqiang, Shao Kuishuang, Sun Yeqing. Application of Water Flea in Experimental Teaching of Aquatic Ecotoxicology Based on Daphnia magna[J]. Asian journal of ecotoxicology, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
shu

模式生物大型溞在水生生态毒理实验教学中的应用

  • 1. 大连海事大学环境科学与工程学院, 环境系统生物学研究所, 大连 116026;

  • 2. 山西大学环境科学研究所, 山西大学环境医学与毒理学研究所, 太原 030006;

  • 3. 国家海洋环境监测中心, 大连 116023

    • 作者简介: 巩宁(1975-),女,博士,教授,研究方向为水生生态毒理学,E-mail:cospar06@dlmu.edu.cn
    通讯作者: 巩宁, E-mail: cospar06@dlmu.edu.cn ; 
  • 基金项目:

    大连海事大学教学改革资助项目（2020Y70）；国家自然科学基金资助项目（41301560）；中央高校基本科研业务费（3132019335）

  • 中图分类号: X171.5

Application of Water Flea in Experimental Teaching of Aquatic Ecotoxicology Based on Daphnia magna

  • 1. College of Environmental Science and Engineering, Institute of Environmental Systems Biology, Dalian Maritime University, Dalian 116026, China;

  • 2. Institute of Environmental Science, Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, China;

  • 3. National Marine Environmental Monitoring Center, Dalian 116023, China

    • Corresponding author: Gong Ning, cospar06@dlmu.edu.cn ; 
  • Fund Project:

  • 摘要: 大型溞（Daphnia magna）因其个体小、繁殖快、易于培养及对污染物敏感等特点，而成为水生毒理学研究中广泛应用的模式物种。本文构建了一套以大型溞为受试动物的水生生态毒理学实验内容和方案，可用于本科生基础性、综合性实验教学。本方案以形态学观察、生理学和毒理学测试为主，也可扩展为包括分子毒理学在内的其他毒性终点的创新性实验。此外，授课教师还可以本方案为基础，对实验内容酌情增减或联合使用，以满足不同层次学生的生态毒理学实验需求。
    Abstract: Daphnia magna is a model species widely used in aquatic toxicology research due to its characteristics such as small size, fast reproduction, easy cultivation and sensitivity to pollutants. Therefore, a set of standard methods for acute and chronic toxicity testing has been established for daphnia. In this paper, based on these standard methods, the experimental teaching system in the aquatic ecotoxicology is constructed with D. magna, which can be used in basic and comprehensive practical or experimental teaching for undergraduates. The program mainly focuses on morphological observation, physiological and toxicological tests. It also can be extended to innovative experiments combining with other toxicity tests including measuring of molecular toxicological biomarkers. The instructor may use the plan as a module, increasing or decreasing of teaching contents, or combining several experiments to meet the needs of students at different levels. This paper hopes to provide reference for experimental teaching of aquatic ecological toxicology and related disciplines.
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  • Daniel D, Dionísio R, de Alkimin G D, et al. Acute and chronic effects of paracetamol exposure on Daphnia magna:How oxidative effects may modulate responses at distinct levels of organization in a model species[J]. Environmental Science and Pollution Research International, 2019, 26(4):3320-3329
    Knapik L F O, Ramsdorf W. Ecotoxicity of malathion pesticide and its genotoxic effects over the biomarker comet assay in Daphnia magna[J]. Environmental Monitoring and Assessment, 2020, 192(5):264
    Danabas D, Ates M, Ertit Tastan B, et al. Effects of Zn and ZnO nanoparticles on Artemia salina and Daphnia magna organisms:Toxicity, accumulation and elimination[J]. Science of the Total Environment, 2020, 711:134869
    Kim H J, Koedrith P, Seo Y R. Ecotoxicogenomic approaches for understanding molecular mechanisms of environmental chemical toxicity using aquatic invertebrate, Daphnia model organism[J]. International Journal of Molecular Sciences, 2015, 16(6):12261-12287
    Organization for Economic Cooperation and Development (OECD). OECD Guideline for Testing of Chemicals. Daphnia sp., Acute Immobilisation Test[S]. Paris:OECD, 2009
    Organization for Economic Cooperation and Development (OECD). OECD Guidelines for Testing of Chemicals. Daphnia magna Reproduction Test[S]. Paris:OECD, 2012
    孟紫强. 生态毒理学[M]. 北京:中国环境出版集团, 2019:226-227
    蒋燮治, 堵南山. 中国动物志节肢动物门甲壳纲淡水枝角类[M]. 北京:科学出版社, 1979:101-102
    杨四秀. 枝角类繁殖生物学研究概况[J]. 水利渔业, 2004, 25(4):12-14

    , 22

    中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 大型溞急性毒性实验方法:GB/T 16125-2012[S]. 北京:中国标准出版社, 2012
    American Society for Testing and Materials (ASTM). Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macro Invertebrates, and Amphibians[S]. West Conshohocken, PA:ASTM, 1989
    International Organization for Standardization (ISO). ISO 6341. Water Quality-Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)-Acute Toxicity Test[S]. Geneva:ISO, 2012
    Kokalj A J, Kunej U, Skalar T. Screening study of four environmentally relevant microplastic pollutants:Uptake and effects on Daphnia magna and Artemia franciscana[J]. Chemosphere, 2018, 208:522-529
    Organization for Economic Cooperation and Development (OECD). OECD Guidelines for the Testing of Chemicals. Freshwater Alga and Cyanobacteria, Growth Inhibition Test[S]. Paris:OECD, 2006
    巩宁. 海洋生物学实验:海洋科学、海洋资源与环境专业[M]. 大连:大连海事大学出版社, 2018:19
    Campbell A K, Wann K T, Matthews S B. Lactose causes heart arrhythmia in the water flea Daphnia pulex[J]. Comparative Biochemistry and Physiology Part B, Biochemistry & Molecular Biology, 2004, 139(2):225-234
    王茜, 郭鹄飞, 王兰. 镉对大型溞摄食能力和相关生理指标的影响[J]. 水生生物学报, 2018, 42(3):616-621

    Wang Q, Guo H F, Wang L. Effect of cadmium on the feeding capacity and physiological status of Daphnia magna[J]. Acta Hydrobiologica Sinica, 2018, 42(3):616-621(in Chinese)

    蔡清洁, 何文辉, 彭自然, 等. pH和盐度对大型溞心率和摄食行为的影响[J]. 上海海洋大学学报, 2017, 26(3):415-421

    Cai Q J, He W H, Peng Z R, et al. Effects of pH and salinity on heart rate and feeding behavior of Daphnia magna[J]. Journal of Shanghai Ocean University, 2017, 26(3):415-421(in Chinese)

    Bownik A, Ślaska B, Dudka J. Cisplatin affects locomotor activity and physiological endpoints of Daphnia magna[J]. Journal of Hazardous Materials, 2020, 384:121259
    中华人民共和国国家质量监督检验检疫总局, 国家标准化管理委员会. 化学品大型溞繁殖试验:GB/T 21828-2008[S]. 北京:中国标准出版社, 2008
    Poynton H C, Taylor N S, Hicks J, et al. Metabolomics of microliter hemolymph samples enables an improved understanding of the combined metabolic and transcriptional responses of Daphnia magna to cadmium[J]. Environmental Science & Technology, 2011, 45(8):3710-3717
    Nagato E G, D'eon J C, Lankadurai B P, et al.1H NMR-based metabolomics investigation of Daphnia magna responses to sub-lethal exposure to arsenic, copper and lithium[J]. Chemosphere, 2013, 93(2):331-337
    Vandegehuchte M B, Lemière F, Janssen C R. Quantitative DNA-methylation in Daphnia magna and effects of multigeneration Zn exposure[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2009, 150(3):343-348
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  • 收稿日期:  2020-01-21
巩宁, 孟紫强, 邵魁双, 孙野青. 模式生物大型溞在水生生态毒理实验教学中的应用[J]. 生态毒理学报, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
引用本文: 巩宁, 孟紫强, 邵魁双, 孙野青. 模式生物大型溞在水生生态毒理实验教学中的应用[J]. 生态毒理学报, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
shu
Gong Ning, Meng Ziqiang, Shao Kuishuang, Sun Yeqing. Application of Water Flea in Experimental Teaching of Aquatic Ecotoxicology Based on Daphnia magna[J]. Asian journal of ecotoxicology, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
Citation: Gong Ning, Meng Ziqiang, Shao Kuishuang, Sun Yeqing. Application of Water Flea in Experimental Teaching of Aquatic Ecotoxicology Based on Daphnia magna[J]. Asian journal of ecotoxicology, 2021, 16(3): 355-360. doi: 10.7524/AJE.1673-5897.20200121001
shu

模式生物大型溞在水生生态毒理实验教学中的应用

    通讯作者: 巩宁, E-mail: cospar06@dlmu.edu.cn ; 
    作者简介: 巩宁(1975-),女,博士,教授,研究方向为水生生态毒理学,E-mail:cospar06@dlmu.edu.cn
  • 1. 大连海事大学环境科学与工程学院, 环境系统生物学研究所, 大连 116026;
  • 2. 山西大学环境科学研究所, 山西大学环境医学与毒理学研究所, 太原 030006;
  • 3. 国家海洋环境监测中心, 大连 116023
  • 收稿日期:  2020-01-21
基金项目:

大连海事大学教学改革资助项目（2020Y70）；国家自然科学基金资助项目（41301560）；中央高校基本科研业务费（3132019335）

摘要: 大型溞（Daphnia magna）因其个体小、繁殖快、易于培养及对污染物敏感等特点，而成为水生毒理学研究中广泛应用的模式物种。本文构建了一套以大型溞为受试动物的水生生态毒理学实验内容和方案，可用于本科生基础性、综合性实验教学。本方案以形态学观察、生理学和毒理学测试为主，也可扩展为包括分子毒理学在内的其他毒性终点的创新性实验。此外，授课教师还可以本方案为基础，对实验内容酌情增减或联合使用，以满足不同层次学生的生态毒理学实验需求。

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