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Hg2+对中国鲎幼体急性毒性及MT的诱导效应

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鲍虞园, 叶国玲, 钟金香, 陈海刚, 李银康, 关杰耀, 颉晓勇. Hg2+对中国鲎幼体急性毒性及MT的诱导效应[J]. 生态毒理学报, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
引用本文: 鲍虞园, 叶国玲, 钟金香, 陈海刚, 李银康, 关杰耀, 颉晓勇. Hg2+对中国鲎幼体急性毒性及MT的诱导效应[J]. 生态毒理学报, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
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Bao Yuyuan, Ye Guoling, Zhong Jinxiang, Chen Haigang, Li Yinkang, Kwan Kit Yue, Xie Xiaoyong. Acute Toxicity of Hg2+ to and Associated Metallothionein Induction in Tachypleus tridentatus Larvae[J]. Asian journal of ecotoxicology, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
Citation: Bao Yuyuan, Ye Guoling, Zhong Jinxiang, Chen Haigang, Li Yinkang, Kwan Kit Yue, Xie Xiaoyong. Acute Toxicity of Hg2+ to and Associated Metallothionein Induction in Tachypleus tridentatus Larvae[J]. Asian journal of ecotoxicology, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
shu

Hg2+对中国鲎幼体急性毒性及MT的诱导效应

  • 1. 中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广州 510300;

  • 2. 上海海洋大学水产与生命学院, 水产种质资源发掘与利用教育部重点实验室, 水产科学国家级实验教学示范中心, 上海水产养殖工程技术研究中心, 上海 201306;

  • 3. 广东省土地调查规划院, 广州 510075;

  • 4. 北部湾大学海洋学院, 钦州 535011

    • 作者简介: 鲍虞园(1995-),男,硕士研究生,研究方向为养殖生物学,E-mail:answerbyy@sina.com
    通讯作者: 颉晓勇, E-mail: xyxie@scsfri.ac.cn
  • 基金项目:

    中央级公益性科研院所基本科研业务费专项资金资助项目（中国水产科学研究院南海水产研究所）（2019TS21）

  • 中图分类号: X171.5

Acute Toxicity of Hg2+ to and Associated Metallothionein Induction in Tachypleus tridentatus Larvae

  • 1. Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;

  • 2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education;National Demonstration Center for Experimental Fisheries, Science Education;Shanghai Engineering Research Center of Aquaculture;College of Fisheries Science and Life Science, Shanghai Ocean University, Shanghai 201306, China;

  • 3. Guangdong Provincial Institute of Land Survey and Plan, Guangzhou 510075, China;

  • 4. College of Ocean, Beibu Gulf University, Qinzhou 535011, China

    • Corresponding author: Xie Xiaoyong, xyxie@scsfri.ac.cn
  • Fund Project:

  • 摘要: 汞是一种以多种形式、广泛而持久地污染水体的重金属，具有高毒性、非生物降解性和生物富集性。为探究汞（Hg）对海洋动物的毒性效应，在实验室条件下，采用静水试验方法进行Hg2+ 96 h胁迫对中国鲎幼体的急性毒性实验，并考察了不同质量浓度（0.005、0.025和0.250 mg·L-1）Hg2+处理对幼年中国鲎体内金属硫蛋白（MT）的影响。结果表明，Hg2+对中国鲎幼体的毒性类型为急性Ⅱ，其24、48、72和96 h半数致死浓度（LC50）分别为15.013、7.084、4.008和2.683 mg·L-1，安全质量浓度为0.473 mg·L-1。在安全浓度范围内，不同浓度的Hg2+均能显著诱导MT的产生（P<0.05），MT含量与Hg2+浓度在一定浓度范围（0～0.025 mg·L-1）和暴露时间（72 h）内表现为正相关；实验组MT含量为对照组的1.152倍～1.716倍。其中以72 h时0.025 mg·L-1组的MT诱导量最高，为（8.93±0.16）ng·mL-1。本研究为天然水环境中幼鲎的安全性评估及渔业部门制定水质标准提供了理论依据。
    Abstract: Mercury is a widespread and persistent waterborne heavy metal pollutant, which possesses highly toxic, non-biodegradable, and bioaccumulative properties. To investigate the potential toxic effects of mercury to marine organisms, the first-instar Tachypleus tridentatus was exposed to Hg2+ at various concentrations (i.e., 0.005, 0.025 and 0.250 mg·L-1) under a 96-h acute experiment. The findings indicated that the Hg2+ median lethal concentration (LC50) for larva at 24 h, 48 h, 72 h and 96 h was 15.013, 7.084, 4.008 and 2.683 mg·L-1, respectively. The no observed adverse effect level (NOAEL) was 0.473 mg·L-1. Hg2+ toxicity level to T. tridentatus larvae was considered medium. Metallothionein (MT) was significantly induced (P<0.05) in all concentration treatments within the NOAEL range. The larval MT concentration was found positively correlated with Hg2+ concentration (0~0.025 mg·L-1) and exposure time (72 h). The larval MT concentration in the treatment group was 1.152~1.716 higher than that in the control group, and was highest under the 0.025 mg·L-1 Hg2+ exposure at 72 h that accounts for (8.93±0.16) ng·mL-1. The results provide theoretical basis to support environmental risk assessment for T. tridentatus larvae and water quality standard establishment by fisheries departments.
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  • 收稿日期:  2019-10-03
鲍虞园, 叶国玲, 钟金香, 陈海刚, 李银康, 关杰耀, 颉晓勇. Hg2+对中国鲎幼体急性毒性及MT的诱导效应[J]. 生态毒理学报, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
引用本文: 鲍虞园, 叶国玲, 钟金香, 陈海刚, 李银康, 关杰耀, 颉晓勇. Hg2+对中国鲎幼体急性毒性及MT的诱导效应[J]. 生态毒理学报, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
shu
Bao Yuyuan, Ye Guoling, Zhong Jinxiang, Chen Haigang, Li Yinkang, Kwan Kit Yue, Xie Xiaoyong. Acute Toxicity of Hg2+ to and Associated Metallothionein Induction in Tachypleus tridentatus Larvae[J]. Asian journal of ecotoxicology, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
Citation: Bao Yuyuan, Ye Guoling, Zhong Jinxiang, Chen Haigang, Li Yinkang, Kwan Kit Yue, Xie Xiaoyong. Acute Toxicity of Hg2+ to and Associated Metallothionein Induction in Tachypleus tridentatus Larvae[J]. Asian journal of ecotoxicology, 2020, 15(6): 300-307. doi: 10.7524/AJE.1673-5897.20191003001
shu

Hg2+对中国鲎幼体急性毒性及MT的诱导效应

    通讯作者: 颉晓勇, E-mail: xyxie@scsfri.ac.cn
    作者简介: 鲍虞园(1995-),男,硕士研究生,研究方向为养殖生物学,E-mail:answerbyy@sina.com
  • 1. 中国水产科学研究院南海水产研究所, 农业农村部南海渔业资源开发利用重点实验室, 广州 510300;
  • 2. 上海海洋大学水产与生命学院, 水产种质资源发掘与利用教育部重点实验室, 水产科学国家级实验教学示范中心, 上海水产养殖工程技术研究中心, 上海 201306;
  • 3. 广东省土地调查规划院, 广州 510075;
  • 4. 北部湾大学海洋学院, 钦州 535011
  • 收稿日期:  2019-10-03
基金项目:

中央级公益性科研院所基本科研业务费专项资金资助项目（中国水产科学研究院南海水产研究所）（2019TS21）

摘要: 汞是一种以多种形式、广泛而持久地污染水体的重金属，具有高毒性、非生物降解性和生物富集性。为探究汞（Hg）对海洋动物的毒性效应，在实验室条件下，采用静水试验方法进行Hg2+ 96 h胁迫对中国鲎幼体的急性毒性实验，并考察了不同质量浓度（0.005、0.025和0.250 mg·L-1）Hg2+处理对幼年中国鲎体内金属硫蛋白（MT）的影响。结果表明，Hg2+对中国鲎幼体的毒性类型为急性Ⅱ，其24、48、72和96 h半数致死浓度（LC50）分别为15.013、7.084、4.008和2.683 mg·L-1，安全质量浓度为0.473 mg·L-1。在安全浓度范围内，不同浓度的Hg2+均能显著诱导MT的产生（P<0.05），MT含量与Hg2+浓度在一定浓度范围（0～0.025 mg·L-1）和暴露时间（72 h）内表现为正相关；实验组MT含量为对照组的1.152倍～1.716倍。其中以72 h时0.025 mg·L-1组的MT诱导量最高，为（8.93±0.16）ng·mL-1。本研究为天然水环境中幼鲎的安全性评估及渔业部门制定水质标准提供了理论依据。

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