有机硒F0代暴露对子代软骨发育的影响
Effect of Dietary Seleno-L-methionine on Cartilage Development in Offspring in Japanese medaka (Oryzias latipes)
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摘要: 硒是生物体必需的微量元素。但硒的过量摄入也许会对动物和人体造成不利的影响。有机硒经由母体对下一世代（F1）的生长发育的影响尤其受到关注。用青鳉鱼作为动物模型，在父本单独暴露、母本单独暴露，以及父本、母本同时暴露于有机硒后，评价了其对F1代发育的影响。结果表明，5 μg·g-1的有机硒可造成F1代存活率降低、畸形率显著升高，特别是造成头部软骨畸形以及下颌突出等形态学的显著变化。SOX9a、SOX9b和Collagen2a1等基因表达与软骨发育密切相关，检测到有机硒可显著诱导这些基因的表达。研究表明，有机硒过量摄入对青鳉鱼F1代的毒性与软骨发育通路障碍相关。Abstract: Selenium (Se) is an essential trace element for organisms. Excessive uptake of selenium may adversely affect animals and humans. Data on the effects of Se on the development of offspring due to maternal transfer are relatively scarce and deserve more attention. In this study, the Japanese medaka (Oryzias latipes) was used as an animal model, and the effects of seleno-L-methionine (Se-Met) on the development of offspring from Se-exposed parents were evaluated. The offspring were obtained from different combinations including Se-exposed male×control female, Se-exposed female×control male, and Se-exposed male×Se-exposed female. The results showed that 5 μg·g-1 of Se-Met in the feed reduced the survival rate of the offspring and increased its deformity rate, especially the morphological changes of head cartilage and mandibular protrusion. The transcriptional expressions of SOX9a, SOX9b, and Collagen2a1 were stimulated by Se-Met, more pronounced in offspring from the parental fish both exposed to Se-Met. Our results demonstrate that excessive Se-Met may cause cartilage development disorders in offspring from Se-exposed parental medaka, which could be explained by the alterations in the transcriptions of genes involved in chondral development.
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Key words:
- Se-Met /
- Oryzias latipes /
- developmental toxicity /
- malformation /
- transgenerational effect /
- F0 /
- F1
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