纳米氧化铝对斑马鱼幼鱼早期运动行为的影响
Effects of Nano-Alumina on Motor Behaviors of Zebrafish Larva
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摘要: 随着纳米技术的迅猛发展，纳米材料的安全性研究具有十分重要的意义。为探讨纳米氧化铝对斑马鱼幼鱼早期运动行为的影响，本研究将受精后6 h(6 hpf)的斑马鱼胚胎随机分成空白对照组(E3培养液)、纳米氧化铝组(12.5、25、50、100 μg·mL-1)。采用6孔板染毒，每组160颗卵，共8个孔，每孔20颗卵/10 mL试液，染毒液更新周期为1 d。观察急性毒性和运动行为。结果显示，各纳米氧化铝组无明显的急性毒性；运动行为检测发现，25、50、100 μg·mL-1纳米氧化铝组受精后6 d幼鱼(6 dpf)黑暗状态下的运动速度、运动距离、趋触性程度较空白对照组均显著下降(P<0.05)；在6 dpf幼鱼对强光刺激的惊恐逃避反射试验中发现，各组幼鱼在光照1 min内运动速度较光照前的黑暗期均明显下降(P<0.05)，但25 μg·mL-1和100 μg·mL-1浓度组在光照时速度下降得更慢(P<0.05)；关闭光源后，各组幼鱼的运动速度都会上升，但25 μg·mL-1和100 μg·mL-1浓度组在打开光源后速度上升得更慢(P<0.05)。上述结果表明，纳米氧化铝可以影响斑马鱼幼鱼早期的运动行为。Abstract: With the rapid development of nanotechnology, the research on the safety of nanomaterial is of great significance. To explore the effects of alumina nanoparticles (AlNPs) on motor behaviors of zebrafish larva, fertilized zebrafish eggs were transferred into 6-well plates, 20 embryos per well, 160 embryos per group, which were exposed to various concentrations of AlNPs suspensions (0, 12.5, 25, 50, 100 μg·mL-1) until larva reach free swimming stage. AlNPs suspensions were renewed daily. The general toxicity on motor behavior were observed. The results showed that hatch rate and mortality rate in AlNPs treated groups had no significant difference compared with that in controls, while larval swimming parameters, including average velocity and total distance moved and thigmotaxis were significantly affected by AlNPs. Strong light stimulation experiments showed that the average velocity of all groups decreased during the light-on period of 1 min and increased after the light was turned off. However, the average velocity of larva in 25 μg·mL-1 and 100 μg·mL-1 group altered much slower (P<0.05). This study suggests that AlNPs can affect motor behaviors in zebrafish larva.
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Key words:
- nano-alumina /
- zebrafish /
- motor behaviors
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