苏丹红I对小鼠卵巢的损伤作用及对Caspase-3和Ki-67表达的影响
Injury and Expression of Caspase-3 and Ki-67 in Ovary of Mice Exposed to SudanⅠ
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摘要: 苏丹红是一种人工合成偶氮染料，可引起肝脏及泌尿系统等多个脏器的肿瘤，但苏丹红对生殖系统的毒性研究较少。本研究以昆明小鼠为受试对象，探讨苏丹红I对小鼠卵巢组织Caspase-3和Ki-67表达的影响。将24只昆明小鼠随机分为：对照组、低剂量(60 mg·kg-1)、中剂量(120 mg·kg-1)、高剂量(240 mg·kg-1) 4组，6只/组。胃灌4周，4周末处死。HE染色观察各组卵巢组织病理变化，免疫组化法检测卵巢组织Caspase-3和Ki-67表达，Real time PCR检测卵巢组织Caspase-3和Ki-67 mRNA表达，Western blotting检测卵巢组织中Ki-67及Caspase-3蛋白表达。与对照组比较，实验组卵巢中Caspase-3阳性细胞率显著升高(P＜0.05)，而Ki-67阳性细胞率显著降低(P＜0.05)，Real time PCR及Western blotting结果与免疫组化法结果一致。本实验发现苏丹红染毒后颗粒细胞的Caspase-3表达增加，且Caspase-3表达呈剂量依赖性，但Ki-67表达受到抑制。Abstract: Sudan is a synthetic azo dye and can cause tumors in multiple organs including liver and urinary system, but the research in reproductive system toxicity is rare. The effect of Sudan I on the expression of Caspase-3 and Ki-67 in the ovarian tissue of mice was studied by gastric gavage. The 24 Kunming mice were randomly divided into 4 groups: control group, low dose (60 mg·kg-1), medium dose (120 mg·kg-1) and high dose (240 mg·kg-1) group. Each group contained 6 mice. After gastric gavage for 4 weeks, the pathological changes were observed by HE staining. The expression of Caspase-3 and Ki-67 in ovary was detected by immunohistochemistry and the mRNA expression and protein expression of Ki-67 and Caspase-3 in mouse ovarian tissues were detected by real time PCR and western blotting. Compared with the control group, the rate of Ki-67 positive cells was significantly lower in high concentration group (P＜0.05). The rate of Caspase-3 positive cells was significantly increased (P＜0.05) in high concentration group and the results of PCR and western blotting were consistent with the immunohistochemical results. The Caspase-3 expression of ovarian granulosa cell was increased after SDI exposure, but the Ki-67 expression was inhibited.
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