福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响

于永利; 陈形; 魏素香; 杨景峰; 董武

doi:10.7524/AJE.1673-5897.20140207001

生态毒理学报

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于永利, 陈形, 魏素香, 杨景峰, 董武. 福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响[J]. 生态毒理学报, 2014, 9(6): 1133-1140. doi: 10.7524/AJE.1673-5897.20140207001

引用本文:

Yu Yongli, Chen Xing, Wei Suxiang, Yang Jingfeng, Dong Wu. Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos[J]. Asian Journal of Ecotoxicology, 2014, 9(6): 1133-1140. doi: 10.7524/AJE.1673-5897.20140207001

Citation:

Yu Yongli, Chen Xing, Wei Suxiang, Yang Jingfeng, Dong Wu. Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos[J]. Asian Journal of Ecotoxicology, 2014, 9(6): 1133-1140. doi: 10.7524/AJE.1673-5897.20140207001

福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响

1. 内蒙古民族大学动物科技学院内蒙古自治区高等学校毒物与动物疾病监控重点实验室, 通辽市 028000;
2. 内蒙古民族大学生命科学学院, 通辽市 028000;
3. 百奥生生物科技公司, 路易斯维尔

作者简介: 于永利(1978-),男,讲师,硕士,主要从事食品与环境卫生学研究。E-mail:707928940@qq.com

基金项目:
内蒙古自治区自然科学基金(Nos.2012MS1203)

国家自然科学基金项目(Nos.30360090.21267015)

教育部新世纪优秀人才支持计划项目(Nos.NCET-04-0262)
中图分类号: X171.5

Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos

1. Inner Mongolia Provincial Key Laboratory for Toxicants and Animal Disease, College of Animal Science and Technology, Inner Mongolia University for Nationalities, TongLiao 028042, China;
2. College of Life Sciences, Inner Mongolia University for Nationalities, TongLiao 028042, China;
3. Bio-Synthesis, Inc. DNA lab Department, Lewisville, USA
Fund Project:

摘要: 福美双是一种二硫代甲氨基甲酸盐类农药,在我国有非常广泛的应用,但其高残留性受到广泛的重视。试验使用斑马鱼胚胎作为动物模型,探讨了福美双对生殖的毒性作用。结果表明福美双虽然没有引起原始生殖细胞表达量的变化,但造成原始生殖细胞移动分布的延迟,以及诱导细胞凋亡。试验表明福美双对胚胎发育以及生殖有显著的影响,进一步说明斑马鱼胚胎可作为农药毒性评价的模型。
- 福美双 /
- 斑马鱼 /
- 胚胎 /
- 原始生殖细胞 /
- 毒性
Abstract: Thiram is one of dithiocarbamate carbamate pesticides, which are the most widely used pesticides in China. However, its high accumulation on organisms has received a lot of attention. In this research, zebrafish embryo was used as an animal model to study the mechanism of thiram induced toxicity in primordial germ cells and relative. The experimental data showed thiram didn't affect the PGC mRNA express amount, but delayed the distribution of PGC by double in situ hybridization and increased apoptosis. The results indicated that thiram has reproductive toxicity, and also suggested zebrafish embryos is an excellent model for assessing the toxicity of pesticides.
- thiram /
- zebrafish /
- embryos /
- primordial germ cells /
- toxicity

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福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响

作者简介: 于永利(1978-),男,讲师,硕士,主要从事食品与环境卫生学研究。E-mail:707928940@qq.com
1. 内蒙古民族大学动物科技学院内蒙古自治区高等学校毒物与动物疾病监控重点实验室, 通辽市 028000;

2. 内蒙古民族大学生命科学学院, 通辽市 028000;

3. 百奥生生物科技公司, 路易斯维尔

收稿日期: 2014-02-07

基金项目:

内蒙古自治区自然科学基金(Nos.2012MS1203)

国家自然科学基金项目(Nos.30360090.21267015)

教育部新世纪优秀人才支持计划项目(Nos.NCET-04-0262)

关键词:

摘要: 福美双是一种二硫代甲氨基甲酸盐类农药,在我国有非常广泛的应用,但其高残留性受到广泛的重视。试验使用斑马鱼胚胎作为动物模型,探讨了福美双对生殖的毒性作用。结果表明福美双虽然没有引起原始生殖细胞表达量的变化,但造成原始生殖细胞移动分布的延迟,以及诱导细胞凋亡。试验表明福美双对胚胎发育以及生殖有显著的影响,进一步说明斑马鱼胚胎可作为农药毒性评价的模型。

English Abstract

Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos

1. Inner Mongolia Provincial Key Laboratory for Toxicants and Animal Disease, College of Animal Science and Technology, Inner Mongolia University for Nationalities, TongLiao 028042, China;
2. College of Life Sciences, Inner Mongolia University for Nationalities, TongLiao 028042, China;
3. Bio-Synthesis, Inc. DNA lab Department, Lewisville, USA

Received Date: 2014-02-07

Fund Project:

Keywords:

Abstract: Thiram is one of dithiocarbamate carbamate pesticides, which are the most widely used pesticides in China. However, its high accumulation on organisms has received a lot of attention. In this research, zebrafish embryo was used as an animal model to study the mechanism of thiram induced toxicity in primordial germ cells and relative. The experimental data showed thiram didn't affect the PGC mRNA express amount, but delayed the distribution of PGC by double in situ hybridization and increased apoptosis. The results indicated that thiram has reproductive toxicity, and also suggested zebrafish embryos is an excellent model for assessing the toxicity of pesticides.

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福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响

作者简介: 于永利(1978-),男,讲师,硕士,主要从事食品与环境卫生学研究。E-mail:707928940@qq.com

Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos

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福美双对斑马鱼早期胚胎原始生殖细胞和形态发育的影响

English Abstract

Thiram Affects Gene Expression of Primordial Germcell and Morphological Development of Zebrafish Embryos

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