氧化乐果对小球藻的毒性研究

宋志慧; 刘冰

doi:10.7524/AJE.1673-5897.20131031001

生态毒理学报

氧化乐果对小球藻的毒性研究

宋志慧, 刘冰. 氧化乐果对小球藻的毒性研究[J]. 生态毒理学报, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

引用本文:

宋志慧, 刘冰. 氧化乐果对小球藻的毒性研究[J]. 生态毒理学报, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

Song Zhihui, Liu Bing. Toxic Effects of Omethoate on Chlorella vulgaris[J]. Asian journal of ecotoxicology, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

Citation:

Song Zhihui, Liu Bing. Toxic Effects of Omethoate on Chlorella vulgaris[J]. Asian journal of ecotoxicology, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

氧化乐果对小球藻的毒性研究

宋志慧,
刘冰

青岛科技大学环境与安全工程学院, 山东青岛 266042

作者简介: 宋志慧(1972-),男,博士,研究方向为生态毒理学,E-mail:songhuey@sina.com

基金项目:
山东省社会科学规划项目(03CJZ03)

山东省高等学校实验技术研究项目(2005-188)
中图分类号: X171.5

Toxic Effects of Omethoate on Chlorella vulgaris

Qingdao University of Science and Technology, Qingdao 266042, China
Fund Project:

摘要: 以小球藻(Chlorella vulgaris)为实验生物,通过研究不同浓度(0、1、5、10、15、20 mg·L^-1)的氧化乐果对小球藻生长发育及EC₅₀,硝酸还原酶活性,叶绿素含量的影响,初步探讨了氧化乐果对小球藻的毒性作用。实验结果表明:氧化乐果对小球藻 96 hEC₅₀值为 4.88 mg·L^-1; 低浓度氧化乐果(1 mg·L^-1)在前 6 天对小球藻细胞增长影响不大,高浓度氧化乐果(大于 5 mg·L^-1)则在初期就表现为抑制,氧化乐果浓度与细胞数量之间表现为负相关(R²=0.65755); 在第 8 天,氧化乐果各浓度组的硝酸还原酶的相对酶活也表现"低促高抑" 作用,但是仍然高于对照组的酶活,整体表现为促进作用; 随着氧化乐果浓度的增加,小球藻叶绿素含量呈现明显降低的趋势。
- 氧化乐果 /
- 小球藻 /
- EC₅₀ /
- 硝酸还原酶活性 /
- 叶绿素含量
Abstract: 8-days sub-chronic toxicity of omethoate to Chlorella vulgaris was tested with nominal concentration of 0、1、5、10、15 and 20 mg·L^-1, respectively. The measurement endpoints during sub-chronic exposure were growth inhibition, and nitrate reductase activity (NRA) in this study. The results showed that the 96 h EC₅₀ of omethoate for C. vulgaris is 4.88 mg·L^-1. The biomass of C. vulgaris has less affect under low concentration (1 mg·L^-1) omethoate exposure, but significant inhibited under high concentration (5 mg·L^-1). The biomass of C. vulgaris was negatively correlated with the concentration of omethoate, with R²of 0.65755. The chlorophyll content was also showed inhibitory effect with the increase of omethoate concentration.
- omethoate /
- Chlorella vulgaris /
- EC₅₀ /
- nitrate reductase activity /
- chlorophyll content

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宋志慧, 刘冰. 氧化乐果对小球藻的毒性研究[J]. 生态毒理学报, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

引用本文:

宋志慧, 刘冰. 氧化乐果对小球藻的毒性研究[J]. 生态毒理学报, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

Song Zhihui, Liu Bing. Toxic Effects of Omethoate on Chlorella vulgaris[J]. Asian journal of ecotoxicology, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

Citation:

Song Zhihui, Liu Bing. Toxic Effects of Omethoate on Chlorella vulgaris[J]. Asian journal of ecotoxicology, 2014, 9(3): 483-489. doi: 10.7524/AJE.1673-5897.20131031001

氧化乐果对小球藻的毒性研究

宋志慧,
刘冰

作者简介: 宋志慧(1972-),男,博士,研究方向为生态毒理学,E-mail:songhuey@sina.com
青岛科技大学环境与安全工程学院, 山东青岛 266042

收稿日期: 2013-10-31

基金项目:

山东省社会科学规划项目(03CJZ03)

山东省高等学校实验技术研究项目(2005-188)

关键词:

摘要: 以小球藻(Chlorella vulgaris)为实验生物,通过研究不同浓度(0、1、5、10、15、20 mg·L^-1)的氧化乐果对小球藻生长发育及EC₅₀,硝酸还原酶活性,叶绿素含量的影响,初步探讨了氧化乐果对小球藻的毒性作用。实验结果表明:氧化乐果对小球藻 96 hEC₅₀值为 4.88 mg·L^-1; 低浓度氧化乐果(1 mg·L^-1)在前 6 天对小球藻细胞增长影响不大,高浓度氧化乐果(大于 5 mg·L^-1)则在初期就表现为抑制,氧化乐果浓度与细胞数量之间表现为负相关(R²=0.65755); 在第 8 天,氧化乐果各浓度组的硝酸还原酶的相对酶活也表现"低促高抑" 作用,但是仍然高于对照组的酶活,整体表现为促进作用; 随着氧化乐果浓度的增加,小球藻叶绿素含量呈现明显降低的趋势。

English Abstract

Toxic Effects of Omethoate on Chlorella vulgaris

Qingdao University of Science and Technology, Qingdao 266042, China

Received Date: 2013-10-31

Fund Project:

Keywords:

Abstract: 8-days sub-chronic toxicity of omethoate to Chlorella vulgaris was tested with nominal concentration of 0、1、5、10、15 and 20 mg·L^-1, respectively. The measurement endpoints during sub-chronic exposure were growth inhibition, and nitrate reductase activity (NRA) in this study. The results showed that the 96 h EC₅₀ of omethoate for C. vulgaris is 4.88 mg·L^-1. The biomass of C. vulgaris has less affect under low concentration (1 mg·L^-1) omethoate exposure, but significant inhibited under high concentration (5 mg·L^-1). The biomass of C. vulgaris was negatively correlated with the concentration of omethoate, with R²of 0.65755. The chlorophyll content was also showed inhibitory effect with the increase of omethoate concentration.