利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化

吴芳; 张振轩; 何欣欣; 朱亚先; 张勇

doi:10.7524/AJE.1673-5897.20131017001

生态毒理学报

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吴芳, 张振轩, 何欣欣, 朱亚先, 张勇. 利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化[J]. 生态毒理学报, 2014, 9(3): 445-452. doi: 10.7524/AJE.1673-5897.20131017001

引用本文:

Wu Fang, Zhang Zhenxuan, He Xinxin, Zhu Yaxian, Zhang Yong. Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay[J]. Asian journal of ecotoxicology, 2014, 9(3): 445-452. doi: 10.7524/AJE.1673-5897.20131017001

Citation:

Wu Fang, Zhang Zhenxuan, He Xinxin, Zhu Yaxian, Zhang Yong. Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay[J]. Asian journal of ecotoxicology, 2014, 9(3): 445-452. doi: 10.7524/AJE.1673-5897.20131017001

利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化

1. 厦门大学环境与生态学院近海海洋环境科学国家重点实验室, 厦门 361102;
2. 厦门大学化学化工学院化学系, 厦门 361005;
3. 漳州职业技术学院, 漳州 363000

作者简介: 吴芳(1980-),女,工程师,研究方向为环境化学,E-mail:wufang@xmu.edu.cn

基金项目:
国家自然科学基金(No.21177102.21075102)

教育部博士点基金(No.20130121130005)
中图分类号: X171.5

Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay

1. State Key Laboratory of Marine Environmental Science(Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361102, China;
2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
3. Zhangzhou Institute of Technology, Zhangzhou 363000, China
Fund Project:

摘要: 将发光细菌毒性测试方法应用于典型多环芳烃生物降解过程的毒性评价,研究了菲和芘单独及混合状态下生物降解过程中的毒性变化。结果表明:溶解态的菲和芘在一定浓度范围内对发光细菌均具有毒性,菲和芘对发光细菌的 10 min-EC₅₀值分别为 1.61×10^-5 mol· L^-1和 2.31×10^-6 mol· L^-1。单组分和混合组分的菲和芘经过微生物降解后,其毒性显著降低。混合组分生物降解体系中由于菲和芘分子之间的互相作用,使其对发光细菌的毒性效应与其单独存在时有差异。生物降解的各阶段,混合组分降解体系的毒性均大于单组分菲或芘降解体系的毒性,且在降解完全的后期,培养液的毒性有增加的趋势。这与菲和芘降解中间产物的积累变化有关。
- 发光细菌 /
- 多环芳烃 /
- 生物降解 /
- 毒性
Abstract: The present study investigated the toxicity variation during biodegradation process of typical PAHs-phenanthrene (Phe) and pyrene (Pyr) -either singly or in a mixture by using luminescent bioassay. The experimental results indicated that dissolved Phe and Pyr were toxicity to luminescent bacteria in a certain range of concentration. The 10 min EC₅₀ of Phe and Pyr to luminescent bacteria were 1.61×10^-5 mol·L^-1 and 2.31×10^-6 mol·L^-1, respectively. The toxicity of Phe and Pyr was significantly reduced after biodegradation process for both single component and multicomponent. Toxicity of multi-component to luminescent bacteria was different from single component due to the interaction of molecules between Phe and Pyr. The toxicity of multi-component biodegradation system was greater than their singly biodegradation system during the overall biodegradation process. In addition the toxicity was increased in later stage of the mixed biodegradation process, possibly due to the generation and changes of metabolites in the biodegradation process.
- luminescent bacteria /
- PAHs /
- biodegradation /
- toxicity

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Citation:

Wu Fang, Zhang Zhenxuan, He Xinxin, Zhu Yaxian, Zhang Yong. Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay[J]. Asian journal of ecotoxicology, 2014, 9(3): 445-452. doi: 10.7524/AJE.1673-5897.20131017001

利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化

作者简介: 吴芳(1980-),女,工程师,研究方向为环境化学,E-mail:wufang@xmu.edu.cn
1. 厦门大学环境与生态学院近海海洋环境科学国家重点实验室, 厦门 361102;

2. 厦门大学化学化工学院化学系, 厦门 361005;

3. 漳州职业技术学院, 漳州 363000

收稿日期: 2013-10-17

基金项目:

国家自然科学基金(No.21177102.21075102)

教育部博士点基金(No.20130121130005)

关键词:

摘要: 将发光细菌毒性测试方法应用于典型多环芳烃生物降解过程的毒性评价,研究了菲和芘单独及混合状态下生物降解过程中的毒性变化。结果表明:溶解态的菲和芘在一定浓度范围内对发光细菌均具有毒性,菲和芘对发光细菌的 10 min-EC₅₀值分别为 1.61×10^-5 mol· L^-1和 2.31×10^-6 mol· L^-1。单组分和混合组分的菲和芘经过微生物降解后,其毒性显著降低。混合组分生物降解体系中由于菲和芘分子之间的互相作用,使其对发光细菌的毒性效应与其单独存在时有差异。生物降解的各阶段,混合组分降解体系的毒性均大于单组分菲或芘降解体系的毒性,且在降解完全的后期,培养液的毒性有增加的趋势。这与菲和芘降解中间产物的积累变化有关。

English Abstract

Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay

1. State Key Laboratory of Marine Environmental Science(Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361102, China;
2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
3. Zhangzhou Institute of Technology, Zhangzhou 363000, China

Received Date: 2013-10-17

Fund Project:

Keywords:

Abstract: The present study investigated the toxicity variation during biodegradation process of typical PAHs-phenanthrene (Phe) and pyrene (Pyr) -either singly or in a mixture by using luminescent bioassay. The experimental results indicated that dissolved Phe and Pyr were toxicity to luminescent bacteria in a certain range of concentration. The 10 min EC₅₀ of Phe and Pyr to luminescent bacteria were 1.61×10^-5 mol·L^-1 and 2.31×10^-6 mol·L^-1, respectively. The toxicity of Phe and Pyr was significantly reduced after biodegradation process for both single component and multicomponent. Toxicity of multi-component to luminescent bacteria was different from single component due to the interaction of molecules between Phe and Pyr. The toxicity of multi-component biodegradation system was greater than their singly biodegradation system during the overall biodegradation process. In addition the toxicity was increased in later stage of the mixed biodegradation process, possibly due to the generation and changes of metabolites in the biodegradation process.

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利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化

作者简介: 吴芳(1980-),女,工程师,研究方向为环境化学,E-mail:wufang@xmu.edu.cn

Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay

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利用发光细菌测试方法评价菲和芘生物降解过程的毒性变化

作者简介: 吴芳(1980-),女,工程师,研究方向为环境化学,E-mail:wufang@xmu.edu.cn 1. 厦门大学环境与生态学院近海海洋环境科学国家重点实验室, 厦门 361102; 2. 厦门大学化学化工学院化学系, 厦门 361005; 3. 漳州职业技术学院, 漳州 363000

English Abstract

Toxicity Variation During Biodegradation of Phenanthrene (Phe) and Pyrene (Pyr) Based on Luminescent Bioassay

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作者简介: 吴芳(1980-),女,工程师,研究方向为环境化学,E-mail:wufang@xmu.edu.cn
1. 厦门大学环境与生态学院近海海洋环境科学国家重点实验室, 厦门 361102;

2. 厦门大学化学化工学院化学系, 厦门 361005;

3. 漳州职业技术学院, 漳州 363000