节织纹螺饲喂富集河豚毒素及相关差异蛋白表达的研究
- 1. 福建省水产研究所, 厦门, 361012;
- 2. 中山大学, 生命科学学院, 广州, 510275
关键词:
- 织纹螺 /
- 河豚毒素 /
- 富集 /
- 双向电泳 /
- 传递
摘要: 我国东南沿海经常有食用含有河豚毒素节织纹螺的中毒事件发生,含有河豚毒素的动物对河豚毒素有很高的耐受能力,并对环境中的河豚毒素具有趋食性。织纹螺可以富集食物中的河豚毒素,但织纹螺体内的与河豚毒素富集相关的蛋白质还不清楚。为此我们收集了无毒的织纹螺,通过河豚毒素富集实验和蛋白质组学分析,发现在有毒织纹螺中有4种蛋白质表达量显著增多,飞行质谱(MALDI-TOF-MS)鉴定分析它们分别是actin、actin2、beta-actin、膜泡ATP合酶B亚基。分析表明这些蛋白可能与河豚毒素在织纹螺体内富集、传递有关。
Tetrodotoxin Enrichment by Feeding Toxin to the Snail Nassarius semiplicata and Proteomic Analysis of Differentially Expressed Proteins
- 1. Fisheries Research Institute of Fujian, Xiamen 361012, China;
- 2. Sun Yat-sen University, School of Life Sciecne, Guangzhou 510275, China
- Received Date:
2014-02-27
Fund Project:
Supported by Overseas funds for Science and Technology and, Marine High-tech Industry Project in Fujian Province([2013]027), and Fund of Xiamen City, China(3502Z20122006).
Abstract: Nassarius poisoning was highly prevalent in the Fujian province of China. Snails contain tetrodotoxin (TTX) which is a potent neurotoxin. TTX-producing animals have higher resistance to the toxin. Snails contain TTX were like to eat diet with TTX. Consumption of TTX diet results in its accumulation in snails. The changes of protein expression profile in TTX-enrichment snail Nassarius semiplicata has not been explored yet. In this study, two-dimensional electrophoresis was used to investigate the protein profile in TTX-enrichment snail Nassarius semiplicata. The results showed that snail Nassarius semiplicata can be attracted by TTX and enrich TTX through food chain. Four proteins including actin, actin 2, β-actin, and vacuolar ATP synthase subunit B partial were observed in this snail. These four proteins may be related to TTX enrichment and transportation in Nassarius semiplicata.
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