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贫营养好氧反硝化菌群的筛选及源水脱氮特性

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周石磊, 黄廷林, 张海涵, 白士远, 何秀秀. 贫营养好氧反硝化菌群的筛选及源水脱氮特性[J]. 环境工程学报, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
引用本文: 周石磊, 黄廷林, 张海涵, 白士远, 何秀秀. 贫营养好氧反硝化菌群的筛选及源水脱氮特性[J]. 环境工程学报, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
shu
Zhou Shilei, Huang Tinglin, Zhang Haihan, Bai Shiyuan, He Xiuxiu. Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
Citation: Zhou Shilei, Huang Tinglin, Zhang Haihan, Bai Shiyuan, He Xiuxiu. Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
shu

贫营养好氧反硝化菌群的筛选及源水脱氮特性

  • 1.

    西安建筑科技大学环境与市政工程学院, 西安 710055

  • 基金项目:

    国家科技支撑计划(2012BAC04B02)

  • 中图分类号: X172

Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water

  • 1.

    School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

  • Fund Project:

  • 摘要: 为了对低浓度氮污染的水体进行净化,通过对微污染水库沉积物进行富集驯化培养,得到好氧反硝化脱氮混合菌。对混合菌进行传代培养得到 10#为效果最好的混合菌。将10#混合菌投加到微污染水库源水中进行投菌实验。实验结果表明:投菌系统的硝氮从初始的(0.38±0.03)mg/L降到(0.07±0.03)mg/L,硝氮的去除率达到(82.30±2.63)%,而空白对照系统的硝氮从初始的(0.34±0.00)mg/L到30 d实验结束变为(0.69±0.01)mg/L;复合菌投菌系统并没有出现亚硝氮的积累;投菌系统总氮从初始的(1.00±0.04)mg/L,到30 d实验结束时总氮下降到(0.52±0.03)mg/L,去除率达到(48.24±2.82)%,但是空白对照系统的总氮从初始的(1.13±0.06)mg/L到30 d结束时为(1.17±0.03)mg/L,基本保持稳定;复合菌系统的好氧反硝化菌菌落数高于空白系统1~2两个数量级。脱氮效果表明,应用复合好氧反硝化菌对于源水脱氮有很好的应用潜力。
    Abstract: To purify micro-polluted source water, mixed denitrification bacterial species were isolated based on enrichment, domestication, and screening processes. The 10# mixed denitrification bacteria with the best nitrogen removal performance were used in a source water experiment. Nitrate in the bacterial system decreased from (0.38±0.03)mg/L to (0.07±0.03)mg/L, and the nitrate removal rate reached (82.30±2.63)%. However, nitrate in the blank system increased from (0.34±0.00)mg/L to (0.69±0.01)mg/L, and no nitrite accumulation was observed. The total nitrogen (TN) of the bacterial system decreased from (1.00±0.04)mg/L to (0.52±0.03)mg/L, while in the blank system, TN was maintained at (1.13±0.06) to (1.17±0.03)mg/L. The numbers of aerobic denitrification bacteria in bacteria system was higher than that in blank system (1 to 2 orders of magnitude). These results suggested that the mixed denitrification bacteria can be used as microbial inoculum for nitrogen removal and have practical applications in the micro-polluted water treatment process.
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  • 收稿日期:  2015-03-05
  • 刊出日期:  2016-06-03
周石磊, 黄廷林, 张海涵, 白士远, 何秀秀. 贫营养好氧反硝化菌群的筛选及源水脱氮特性[J]. 环境工程学报, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
引用本文: 周石磊, 黄廷林, 张海涵, 白士远, 何秀秀. 贫营养好氧反硝化菌群的筛选及源水脱氮特性[J]. 环境工程学报, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
shu
Zhou Shilei, Huang Tinglin, Zhang Haihan, Bai Shiyuan, He Xiuxiu. Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
Citation: Zhou Shilei, Huang Tinglin, Zhang Haihan, Bai Shiyuan, He Xiuxiu. Isolation of oligotrophic aerobic denitrifiers and its characteristics of nitrogen removal in source water[J]. Chinese Journal of Environmental Engineering, 2016, 10(6): 2955-2961. doi: 10.12030/j.cjee.201501176
shu

贫营养好氧反硝化菌群的筛选及源水脱氮特性

  • 1. 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 收稿日期:  2015-03-05
基金项目:

国家科技支撑计划(2012BAC04B02)

摘要: 为了对低浓度氮污染的水体进行净化,通过对微污染水库沉积物进行富集驯化培养,得到好氧反硝化脱氮混合菌。对混合菌进行传代培养得到 10#为效果最好的混合菌。将10#混合菌投加到微污染水库源水中进行投菌实验。实验结果表明:投菌系统的硝氮从初始的(0.38±0.03)mg/L降到(0.07±0.03)mg/L,硝氮的去除率达到(82.30±2.63)%,而空白对照系统的硝氮从初始的(0.34±0.00)mg/L到30 d实验结束变为(0.69±0.01)mg/L;复合菌投菌系统并没有出现亚硝氮的积累;投菌系统总氮从初始的(1.00±0.04)mg/L,到30 d实验结束时总氮下降到(0.52±0.03)mg/L,去除率达到(48.24±2.82)%,但是空白对照系统的总氮从初始的(1.13±0.06)mg/L到30 d结束时为(1.17±0.03)mg/L,基本保持稳定;复合菌系统的好氧反硝化菌菌落数高于空白系统1~2两个数量级。脱氮效果表明,应用复合好氧反硝化菌对于源水脱氮有很好的应用潜力。

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